Not available outside of the UK & Ireland.

Application

SpecificityOrange fluorescence detection reagents are often used with Cy^®3 filter.Application NotePrimary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Duolink^® in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink^® projectsView full Duolink^® product list

Features and Benefits

Multicolor Duolink^® proximity ligation assay (PLA^®) is an application of the Duolink^® technology that allows for endogenous, simultaneous detection of up to 4 protein interactions, post-translational modifications, or protein expression levels (including low abundant protein) in fixed cells and tissue samples. Follow the Duolink^® PLA Probemaker Guide for Multicolor Detection and the Duolink^® PLA Multicolor Detection protocol to use this product. Visualize and detect multiple proteins events in one experiment. No overexpression or genetic manipulation required High specificity (fewer false positives) Detection of low abundant proteins due to rolling circle amplification Relative quantification possible No special equipment needed Faster and more simple than FRET Increased accuracy compared to co-IP Publication-ready resultsComponentsDuolink^® PLA Multicolor Probemaker Kit - Orange contains all the necessary reagents to perform the conjugations of Orange Oligo F and Orange Oligo G directly to primary antibodies. The resulting PLA probes (Orange F and Orange G) must be used together and cannot be paired with any other Duolink^® PLA probe. This product is comprised of the following: Orange Oligo F DUO86030F Orange Oligo G DUO86030G Conjugation Buffer DUO82033 Stop Reagent DUO82034 Storage Solution DUO82035See datasheet for more information.To perform a Multicolor Duolink^® PLA experiment primary antibodies (PLA, IHC, ICC or IF validated) that recognize protein targets of interest are required. Duolink^® PLA Multicolor Probemaker-generated probes (Red A/Red B, Green C/Green D, Orange F/Orange G, and/or FarRed H/FarRed I) are used in combination to perform the Multiplex experiment.The reagents in the Duolink^® PLA Multicolor Reagent Pack are required to perform the amplification and detection of the resulting PLA probes. Required additional reagents include Duolink^® Wash Buffers and Mounting Medium.

Legal Information

Cy is a registered trademark of Cytiva

Duolink is a registered trademark of Sigma-Aldrich Co. LLC

PLA is a registered trademark of Sigma-Aldrich Co. LLC