Not available outside of the UK & Ireland.

Application

The dissipation of the mitochondrial electrochemical potential gradient (δψ) is known as an early event in apoptosis. This kit offers a fast and convenient method for the detection of changes in mitochondrial inner-membrane electrochemical potential in living cells using the cationic, lipophilic dye, JC-1.

Features and Benefits

Kit offers: A fast, simple, and convenient method for staining and assaying mitochondria intactness Contains all the reagents required for the detection of changes mitochondrial inner-membrane electrochemical potential Contains the antibiotic valinomycin, which can be used as a control agent that prevents JC-1 aggregation The shift in fluorescence is clearly detectable in both green and red channels Suitable for adherent cells as well as cells in suspension Has been tested with Jurkat, U-937, HeLa, NIH3T3 cells The fluorescence signal can be observed by fluorescence microscopy or measured by flow cytometry

General description

In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) prevents the accumulation of the JC-1 dye in the mitochondria and thus, the dye is dispersed throughout the entire cell leading to a shift from red (JC-1-aggregates) to green fluorescence (JC-1 monomers). The fluorescence of the cells stained with this kit may be observed by fluorescence microscopy or measured by fluorimetric and flow cytometry analysis.