ANTI-FLAG M2 Affinity Gel, purified immunoglobulin, buffered aqueous glycerol solution

Code: a2220-4x25ml D2-231

Not available outside of the UK & Ireland.

Application

Anti-FLAG^® M2 affinity gel has been used for western blotting, immunoprecipitation and for the purification of FLAG fusion proteins.Browse additio...

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Not available outside of the UK & Ireland.

Application

Anti-FLAG^® M2 affinity gel has been used for western blotting, immunoprecipitation and for the purification of FLAG fusion proteins.Browse additional application references in our FLAG^® Literature portal.

General description

Anti-FLAG M2 Affinity gel is a mouse monoclonal antibody that is covalently attached to agarose. The antibody binds FLAG at the N-terminal, Met-N-terminal, C-terminal and internal locations of fusion proteins. Binding is calcium-independent.Elution - FLAG^® peptide, Glycine, pH 3.5, 3x FLAG^® peptide

Immunogen

DYKDDDDK

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Physical form

Suspension in buffered saline containing azide as preservative and 50% glycerol

analyte chemical class(es)	proteins (FLAG^® tag, 3x FLAG^®, DYKDDDDK tag)
antibody form	purified immunoglobulin
antibody product type	primary antibodies
capacity	>0.6 mg/mL, resin binding capacity (FLAG-BAP)
clone	M2, monoclonal
conjugate	agarose conjugate
form	buffered aqueous glycerol solution
isotype	IgG1
matrix	(4% agarose bead; 45-165µm bead size)
Quality Level	200
shipped in	wet ice
storage temp.	−20°C
technique(s)	affinity chromatography: suitable (FLAG^® peptide, Glycine, pH3.5, 3x FLAG^® peptide), immunoprecipitation (IP): suitable