Not available outside of the UK & Ireland.

Analysis Note

ASB-14 (amidosulfobetaine-14), a membrane solubilizing zwitterionic detergent (medium chain amidosulfobetaine surfactant), may be used in protein and macromolecule research for the preparative extraction and solubilization of proteins for separation and analysis by methods such as 2D-electrophoresis. ASB-14 may be used in tissue engineering for antigen removal (AR) for xenogeneic scaffold generation. ASB-14 may be used to develop and study zwitterionic micelles.

Application

ASB-14 has been used as a component of rehydration buffer for proteomic sample preparation from Leishmania donovani.

Zwitterionic detergent. Useful for solubilization of proteins, including membrane proteins, for 2D electrophoresis.

Biochem/physiol Actions

ASB-14 is useful in proteasome extraction especially for the membrane associated proteins. Along with CHAPS (3-(3-cholamidopropyl)dimethylammonio)-1-propanesulfonate), it is employed in protein solubilization even in the presence of thiourea.

General description

ASB-14 comprises alkyl tails and amidosulfobetaine headgroup.

Packaging

1 g in poly bottle