Not available outside of the UK & Ireland.

Components

2 x 100 mlBugBuster Protein Extraction Reagentr> 10,000 UBenzonase Nuclease, purity >90%r> 10 mlGST Bind Resinr> pkg/4Chromatography Columnsr> 2 x 100 ml10X GST Bind/Wash Buffer r> 40 ml10X Glutathione Reconstitution Bufferr> 1 gGlutathione, Reduced

General description

The BugBuster^® GST•Bind﹤TMSYMBOL>﹤/TMSYMBOL> Purification Kit combines the GST•Bind Resin, GST•Bind Buffer Kit reagents and BugBuster Protein Extraction Reagent for convenient preparation of soluble cell extracts and affinity purification of GST•Tag™ fusion proteins. BugBuster Protein Extraction Reagent is formulated for the gentle disruption of the cell wall of E. coli, resulting in the liberation of soluble protein. Cells are harvested by centrifugation as usual, followed by suspension in BugBuster Reagent. During a brief incubation, soluble proteins are released. The extract is clarified by centrifugation, which removes cell debris and insoluble proteins. The clarified extract is ready to apply to GST•Bind Resin.

Affinity purification of GST fusion proteins

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

1 kit in Glass bottle

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)