Not available outside of the UK & Ireland.

Application

Human recombinant HDAC3 (His-tag) produced in insect cells.

Research CategoryEpigenetics & Nuclear Function

HDAC deacetylase assays

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Unlike other class I HDAC enzymes, HDAC3 is found in co-repressor complexes with the SMRT/N-CoR proteins. HDAC3 contains a unique domain structure which includes both nuclear localization and nuclear export sequences. HDAC3 has been implicated in cancer, as HDAC inhibitors produce anti-proliferative effects in various cancer cells. HDAC3 may also play a role in heart disease, which may involve deacetylation of MEF2.

Human recombinant HDAC3 (His-tag) produced in insect cells

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

In liquid containing 50 mM Tris, pH 8.0, 138 mM NaCl and 10% glycerol. Frozen solution.

Format: Purified

Storage and Stability

Store at -80°C. The enzyme is stable on ice for 30 to 60 min, but may lose activity with dilution and/or prolonged storage on ice. It is recommended that thawing and addition of the enzyme to assay wells be done within as short a time as possible before start of the assay and that the thawed enzyme be kept on ice throughout. Best performance will be obtained by adding the chilled, undiluted enzyme directly to pre-warmed buffer and proceeding immediately to the addition of pre-warmed substrate. The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the HDAC3 into separate tubes before storing at -80°C.

Unit Definition

Specific Activity: 1U = 1 pmol/min at 37°C using a standard flourometric activity assay to measure deacetylation activity