For the rapid extraction and purification of high-quality plasmid DNA from medium scale cultures for use in transfection, sequencing, and enzymatic amplification and modification. High-column capacity allows for a proportional yield for input sample volume of 25 to 50 ml (for high copy number plasmid) and up to 500 ml for low copy number plasmid. Provides plasmid DNA with excellent purity and low endotoxin levels. Increased ease of use due to minimal pipetting volume changes, color-coded kit components, and pre-equilibrated columns.