For applications requiring highly accurate amplification, Accuris offers a range of high fidelity PCR options. The proofreading enzymes are modified for better sensitivity and higher activity, and this ensures more reproducible performance with a wide range of targets. For applications requiring highly accurate amplification, and full control of enzyme concentration, choose High Fidelity DNA polymerase kit. The polymerase enzyme has been genetically modified for better solubility and higher activity across a broad range of ionic conditions, and will amplify a wide range of targets, including those that are GC or AT rich as well as crude DNA samples. A 3′-5′ proofreading exonuclease activity and an error rate of 4.55×10-7 makes Accuris High Fidelity DNA Polymerase the perfect partner for cloning applications. The included 5X buffer contains an optimised concentration of dNTPs and enhancers for compatibility with a variety of amplicons up to 10kb in length.

• 2 tube formulation
• Leaves a blunt end for blunt end vector cloning
• 50x fidelity of native Taq
• Ideal for complex targets up to 10kb