Not available outside of the UK & Ireland.

Application

Oligo(dT)23, Anchored has been used in the synthesis of cDNA for real-time quantitative polymerase chain reaction (PCR).

The Anchored Oligo(dT)₂₃ Primers have 23 thymidine residues and one G, C, or A residue (the anchor) at the 3′ end. This anchor ensures the oligo(dT)₂₃ primer binds at the beginning of the message such that there are no long regions of unusable sequence. Anchored oligo(dT)₂₃ primers may provide an advantage over standard oligo(dT) primers when generating cDNA from poly(A)+ RNA.

Features and Benefits

Oligo(dT)23, Anchored primers can be used in conjunction with random nonamers (Product No. R 7647) when preparing cDNA libraries, when sequence information is incomplete or absent, or other instances where specific primers are not useful Can be used as an alternative to reverse transcription primers for first-strand cDNA synthesis, cDNA library construction, and other applications The anchored oligo(dT)23 primers will not affect PCR after transcription due to their decreased ability to prime at increased temperatures (up to 65 °C)

General description

Oligo(dT)23, Anchored primers are used to prime mRNA with a poly(A) tail for cDNA synthesis. The primers have 23 thymidine residues and one G, C, or A residue (the anchor) at the 3′ end. This anchor ensures that the oligo(dT) primer binds at the very beginning of the message and there is not a long region of the unusable sequence.

Packaging

0.1 mL in poly bottle