Not available outside of the UK & Ireland.

Application

MystiCq^® microRNA SYBR^® Green qPCR ReadyMix^™ has been used in a quantitative real-time polymerase chain reaction (qPCR) to quantify miRNA expression levels in different cell lines.

Features and Benefits

Exclusively for use with the MystiCq microRNA qPCR Assay Primers, Control Primers, and Universal PCR primer 2X concentrated for easy reaction setup with hot-start Taq, dNTPs and optimized buffer Passive reference dye (if required) included at the optimal concentration This master mix can readily amplify fragments between 400 and 500 bp; however, for best results, amplicon size should be limited to 80 - 200 bp

General description

The MystiCq^® microRNA SYBR^® Green qPCR ReadyMix^™ is one of the integral components of the MystiCq microRNA qPCR Assay System. This master mix is a specially formulated 2X concentrate with an antibody-based hot-start Taq DNA polymerase and all the optimized components needed for highly specific, reliable, and reproducible microRNA qPCR assays. Simply add the specific MystiCq microRNA qPCR Assay Primer, the MystiCq Universal PCR primer, and cDNA template generated by the MystiCq microRNA cDNA Synthesis Kit and you′re ready to go. Because there are different methods used for fluorescent normalization on different real-time PCR instruments, there is a MystiCq microRNA SYBR Green qPCR ReadyMix available for your platform.

Legal Information

SYBR is a registered trademark of Life Technologies

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

MystiCq is a registered trademark of Qiagen Beverly Inc.

ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

Other Notes

MystiCq^® microRNA SYBR^® Green qPCR ReadyMix^™, ROX^™, is compatible with the following platforms: Applied Biosystems 5700, Applied Biosystems 7000, Applied Biosystems 7300, Applied Biosystems 7700, Applied Biosystems 7900, Applied Biosystems 7900 HT Fast, Applied Biosystems 7900 HT, Applied Biosystems StepOnePlus, Applied Biosystems StepOne.

Packaging

Default reaction volume is 50 µL100RXN is packaged as 2 X 1.25 mL500RXN is packaged as 10 X 1.25 mL2000RXN is packaged as 1 X 50 mL