Not available outside of the UK & Ireland.

Application

JumpStart^™ Taq ReadyMix^™ for High Throughput Quantitative PCR has been used: For quantitative polymerase chain reaction (qPCR) of cDNA templateAs a component of PCR reaction mix for amplification of DNA extracted from peripheral blood leucocytesFor qPCR amplifications using SYBRFor multiplex PCRFor reduction of primer dimers

Features and Benefits

JumpStart^™ Taq DNA polymerase prevents amplification of non-specific products, resulting in increased efficiency and higher target yield When performing large number of PCR reactions, JumpStart^™ Taq ReadyMix^™ reduces preparation time and the risk of contamination from multiple pipetting steps This master mix allows consistent batch-to-batch and reaction-to-reaction performance Internal Reference Dye is provided for reaction normalization

General description

JumpStart^™ Taq ReadyMix^™ For High Throughput Quantitative PCR combines the performance enhancements of JumpStart Taq Antibody for hot start PCR with the convenience of an easy-to-use reaction mixture that incorporates the internal reference dye for ABI and other real time instrument applications. This 2× concentrate master mix contains JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and reaction buffer. Simply add an equal volume of the 2× ReadyMix to a 2× mixture DNA template, primers and fluorescent probe.To prepare a typical qPCR reaction, mix 25 µL of JumpStart ReadyMix Taq, fluorescent probe, desired amount of magnesium chloride (above 1.5 mM final concentration), template DNA, and primers in a final volume of 50 µL. Reaction volumes can be scaled down, if desired.

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Eppendorf is a registered trademark of Eppendorf AG

Packaging

Default reaction volume is 50 µL20RXN is packaged as 1 X 500 µL400RXN is packaged as 1 X 10 mL2000RXN is packaged as 1 X 50 mL

Quantity

Use 2.5 units per reaction.