Not available outside of the UK & Ireland.

Application

Extract-N-Amp^™ Blood PCR Kit has been used to extract DNA from genomes and dried blood spot samples. It has also been used for polymerase chain reaction (PCR).

Features and Benefits

Efficient 8 minute prep allows greater speed and throughput No need for any type of purification, organic extraction, centrifugation or alcohol precipitation Simple, 3 step procedure with no special equipment required Hot start antibody included for highly specific PCR amplification of genomic DNA Compatible with multiple format (single tube or 96-well plate) Can be used with whole blood or blood cards Extract stable at 4 °C for at least 6 months

General description

The Extract-N-Amp^™ Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by direct PCR. This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix^™, specially formulated for amplification directly from the extract. This formulation uses an antibody based hot start, for specific amplification.

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Other Notes

For additional information, please see www.sigma-aldrich.com/extract-n-amp.

Principle

Genomic DNA is extracted from 10 µl of whole blood by simply adding the Extraction Solution and incubating for 5 minutes at room temperature. The Neutralization Solution is added to the extract to counteract inhibitory substances prior to PCR. A portion of the DNA extract is then added to the specially formulated PCR ReadyMix.