Not available outside of the UK & Ireland.

Application

Quantitative RT-PCR ReadyMix^™ has been used in the quantification of messenger RNA (mRNA) levels of specific expressed genes by quantitative reverse-transcription polymerase chain reaction (RT-qPCR).

Features and Benefits

The master mix allows consistency and reproducibility from one reaction to the next Reduced risk of contamination from multiple pipetting steps Reduced set-up time as compared to manual or wax Hot Start methods JumpStart^™Taq Polymerase reduces primer-dimer and non-specific product formation Broad instrument compatibility Includes a separate ROX reference dye vial for reaction normalization

General description

Quantitative RT-PCR ReadyMix^™ combines Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and JumpStart^™ Taq DNA polymerase in a one-step RT-PCR kit designed for the measurement of gene expression. This convenient 2X master mix includes M-MLV RT, JumpStart^™ Taq DNA polymerase, 99% pure deoxynucleotides, buffer, glass passivator, and stabilizers. The JumpStart^™ Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. This kit has been formulated for fluorogenic hybridization probe-based detection methods.

Legal Information

This product is for research use only.

JumpStart is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Eppendorf is a registered trademark of Eppendorf AG

Packaging

1 kit sufficient for 250 reactions at 20 µL each or for 100 reactions at 50 µL each.