Not available outside of the UK & Ireland.

Application

Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart^® One-Step Probe RT-qPCR ReadyMix^™ does not contain an internal reference dye.Inhibitor Resistant Genotyping PCR ReadyMix^™ has been used as a component of the reaction mixture for detection of leucoanthocyanidin reductase(PaLAR3) alleles in a genotyping assay and multiplex PCR for amplification of microsatellite.

Components

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, insert blue qPCR dye, and stabilizers.packaging:250 reactions* = 2 X 1.25 mL tubes1250 reactions* = 10 X 1.25 mL tubes5000 reactions* = 1 X 50 mL tube*number of reactions based on a 20uL volume

Features and Benefits

Assay results in as little as 33 minutes Highly efficient and sensitive real-time PCR results Little/no optimization required Compatible with both fast and conventional PCR cycling protocols

General description

Inhibitor Resistant Genotyping PCR Readymix is a 2X concentrated ready-to-use master mix for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan^® hydrolysis probes. Inhibitor Resistant Genotyping PCR Readymix contains all required reaction components, except primers, probes, and DNA template. The light blue color of the inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with reporter dye fluorescence or affect the stability of the product.A key component of Inhibitor Resistant Genotyping PCR ReadyMix is an ultra-pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification.

Legal Information

Chromo4 is a trademark of Bio-Rad Laboratories, Inc.

Mastercycler is a registered trademark of Eppendorf AG

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

MiniOpticon is a trademark of Bio-Rad Laboratories, Inc.

Opticon is a trademark of Bio-Rad Laboratories, Inc.

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

CFX384 is a trademark of Bio-Rad Laboratories, Inc.

SmartCycler is a registered trademark of Cepheid, Inc.

Eppendorf is a registered trademark of Eppendorf AG

LightCycler is a registered trademark of Roche

CFX96 is a trademark of Bio-Rad Laboratories, Inc.

Rotor-Gene is a registered trademark of Qiagen GmbH

Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc.

KiCqStart is a registered trademark of Qiagen Beverly Inc.

Other Notes

Compatible instruments include: Bio-Rad^® CFX384^™ Bio-Rad CFX96^™ Bio-Rad MiniOpticon^™ Bio-Rad/MJ Chromo4^™ Bio-Rad/MJ Opticon 2 Bio-Rad/MJ Opticon^™ Cepheid SmartCycler^® Eppendorf Mastercycler^® ep realplex Eppendorf Mastercycler ep realplex2 s Illumina Eco qPCR Qiagen/Corbett Rotor-Gene^® 3000 Qiagen/Corbett Rotor-Gene 6000 Qiagen/Corbett Rotor-Gene Q Roche LightCycler^® 480