Not available outside of the UK & Ireland.

Application

The purified PCR product is ready for a variety of downstream applications including sequencing, restriction digest, ligation and microarray analysis.

Features and Benefits

Complete removal of primers and primer-dimers Innovative wash plate minimizes risk of cross-contamination Suitable for processing under vacuum and centrifugation Time-saving parallel clean-up of PCR products

General description

GenElute 96 Well PCR Clean-Up Kit allows for high throughput purification of PCR products. The kit provides the necessary reagents for purification of highly pure PCR products. The DNA recovery is 75-90% for fragments of 100 to 10,000 bp and removes primers, primer-dimers, nucleotides, salts and polymerase.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Principle

Once the PCR reaction is complete, the reaction volume is adjusted and the Binding Solution is added. The samples are transferred to the Binding Plate where the PCR reaction is captured on the silica membrane. The bound PCR product is washed several times to remove primers, primer-dimers, salts, nucleotides and polymerases. Finally the purified PCR product is eluted and ready for immediate use in downstream applications.