Not available outside of the UK & Ireland.

Application

M-MLV Reverse Transcriptase has been used:for the preparation of cDNA libraries or for first strand cDNA synthesisfor use in a 2-step RT-PCR assay in quantitative realtime-polymerase chain reaction (RT-qPCR) in reverse transcription

Features and Benefits

Thermostable reverse transcriptase active at 37 °C. Can be used to generate first strand cDNA of up to 7 kb.

General description

Moloney murine leukemia virus (M-MLV ) reverse transcriptase enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. MoMLV RT is made up of 671 amino acid residues. It is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand.

Legal Information

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

Packaging

Supplied with 10× M-MLV reverse transcriptase buffer containing DTT.

Preparation Note

The enzyme is purified from Escherichia coli expressing the pol gene of M-MLV on a plasmid.

Unit Definition

One unit incorporates 1 nmol of TTP into acid precipitable material in 10 min. at 37 °C using poly(A):oligo dT as a template:primer.