l6544-2000rxn

Not available outside of the UK & Ireland.

Application

LuminoCt^® SYBR^® Green qPCR ReadyMix^™ has been used for fast quantitative polymerase chain reaction (qPCR) and quantitative RT-PCR amplifications. It has also been used in qPCR for measuring gene expression.

Features and Benefits

Ideal for performing high-throughput, quantitative PCR applications The master mix allows consistency from one reaction to the next Reduced preparation time and reduced risk of contamination from multiple pipetting steps The hot start mechanism using the JumpStart Taq antibody prevents amplification of non-specific products, resulting in increased efficiency and higher target yield LuminoCt^® ReadyMixes require the use of small amplicons (﹤200bp) for optimal results This master mix is compatible with commercial primer sets, including TaqMan^® assays

Assay results in as little as 25 minutes Unsurpassed accuracy, precision, and sensitivity Virtually eliminates the need to optimize assay parameters

General description

LuminoCt^® SYBR^® Green qPCR ReadyMix is ready to use master mix that combines the performance enhancements of our JumpStart^™ Taq antibody for hot start. Extensive design and validation of the ReadyMix chemistry has virtually eliminated the need for optimization when LuminoCt is used in conjunction with properly designed primers. Inclusion of JumpStart^™ Taq antibody in the ReadyMix eliminates polymerase activity at ambient temperatures without causing the performance issues associated with chemically modified hot-start Taq. This allows for very rapid activation of the Taq and delivers unparalleled assay sensitivity, while allowing for benchtop reaction setup. Kit is designed to perform SYBR Green-based qPCR assays on commonly available real-time instrument platforms ReadyMix requires the addition of reference dye (provided in kit) when being used on real-time instruments that require ROX passive reference dye for normalization of qPCR assays Kit is not compatible with qPCR instruments that utilize glass capillary tubes

Legal Information

SYBR is a registered trademark of Life Technologies

TaqMan is a registered trademark of Roche Molecular Systems, Inc.

SabreLite is a registered trademark of Pelican Products, Inc.

JumpStart is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Eppendorf is a registered trademark of Eppendorf AG

LuminoCt is a registered trademark of Sigma-Aldrich Co. LLC

Other Notes

At a minimum, use software to design primers for assays and ensure that qPCR amplicons are ﹤200 bp Sigma recommends designing primers and probes with Beacon Designer software To order primers and probes from Sigma, visit: www.sigma.com/oligos

Packaging

Default reaction volume is 50 µL100RXN is packaged as 1 X 2.5 mL500RXN is packaged as 1 X 12.5 mL2000RXN is packaged as 1 X 50 mL

application(s)	agriculture
color	colorless
compatibility	for use with Roche LightCycler 480, for use with ABI 7700, for use with Eppendorf^® Mastercycler ep realplex, for use with ABI StepOne, for use with Bio-Rad CFX384, for use with Strategene Mx4000, for use with Strategene Mx3005P, for use with Bio-Rad CFX96, for use with Eppendorf^® Mastercycler ep realplex2 s, for use with ABI 7000, for use with Strategene Mx3000P, for use with ABI ViiA 7, for use with ABI 7300, for use with Qiagen Corbett Rotor-Gene Q, for use with ABI StepOnePlus, for use with Qiagen Corbett Rotor-Gene 3000, for use with ABI 7900, for use with Bio-Rad MJ Chromo4, Bio-Rad MyiQ, for use with Bio-Rad MJ Opticon 2, Bio-Rad iCycler iQ, for use with Bio-Rad MJ Opticon Cepheid SmartCycler, for use with ABI 7500, for use with ABI 5700, for use with ABI 7900 Fast, for use with Bio-Rad MJ Opticon, for use with Illumina Eco qPCR, for use with Bio-Rad MiniOpticon, for use with ABI 7900 HT, for use with Qiagen Corbett Rotor-Gene 6000, for use with ABI 7500 Fast, Bio-Rad iQ 5
detection method	SYBR^® Green
feature	hotstart, dNTPs included
form	liquid
input	purified DNA
Quality Level	200
shipped in	wet ice
storage temp.	−20°C
technique(s)	qPCR: suitable
usage	sufficient for 100 reactions, sufficient for 2000 reactions, sufficient for 500 reactions