Not available outside of the UK & Ireland.

Application

KAPA Taq EXtra HotStart^® ReadyMix^™ has been used for: PCR of amplicons to be analyzed by agarose gel electrophoresis PCR of short and medium length targets (﹤5 kb) Long-range PCR (5 – 15 kb) PCR with limiting amounts of template DNA

Features and Benefits

KAPA Taq EXtra HotStart^® is a blend of KAPA Taq HotStart and a proofreading DNA polymerase Suitable for short-, medium- and long-range PCR, and PCR with low amounts of template DNA Fidelity is 2–4 times better than Taq For short amplicons, replace Taq DNA polymerase for improvements in yield and/or fidelity. Amplify mid- to long-range targets with high yield and sensitivity. 2X ReadyMix with dye contains two inert tracking dyes to allow loading of PCR products directly onto agarose gels for analysis.

General description

KAPA Taq EXtra HotStart^® ReadyMix with dye (2X) is a ready-to-use cocktail containing all components for short- to long-range PCR, except primers and template. The 2X ReadyMix contains KAPA Taq EXtra HotStart DNA Polymerase (0.675 U per 25 µL reaction), KAPA Taq EXtra Buffer (1X), dNTPs (0.3 mM of each dNTP at 1X), MgCl₂ (2 mM at 1X) and stabilizers. The 2X ReadyMix also contains two inert tracking dyes to enable direct loading of PCR products onto agarose gels for analysis by electrophoresis, without the need to add a DNA loading solution.

Legal Information

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

HOTSTART is a registered trademark of Molecular BioProducts, Inc.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Preparation Note

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage.

Quality

Each batch of KAPA Taq EXtra HotStart^® DNA Polymerase is confirmed to contain ﹤2% contaminating protein (Agilent Protein 230 Assay). The 2X KAPA Taq EXtra HotStart ReadyMix is subjected to stringent quality control tests, is free of contaminating exo- and endonuclease activity, and meets strict requirements with respect to DNA contamination levels.