Not available outside of the UK & Ireland.

Application

KAPA dNTP Mix has been used in:Standard PCRHigh fidelity PCR Quantitative PCR End-repair or A-tailingcDNA synthesis DNA sequencing DNA labelingMicroarraysKAPA dNTPs are suitable for use in any molecular biology application requiring high-quality dNTPs.

Features and Benefits

KAPA dNTPs are available as individual dNTPs or as a ready-to-use dNTP Mix. KAPA dNTP Mix is supplied with KAPA Taq, KAPA2G Fast, KAPA2G Robust, KAPA LongRange and KAPA HiFi PCR Kits. KAPA dNTPs are subjected to strict quality control to ensure high performance, particularly with B-family polymerases that are sensitive to poisoning by uracil. Compatible with any commercially available DNA polymerases.

General description

KAPA dinucleotide triphosphates (dNTPs) provide high-quality, molecular, premium grade dinucleotide phosphates (dNTPs) mainly used for polymerase chain reaction (PCR).

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Preparation Note

Always ensure that the product KAPA dNTP Mix has been fully thawed and mixed before use. KAPA dNTPs may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that the sample has been handled carefully and not contaminated, the samples are not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days). Long-term storage at room temperature and 4°C is not recommended. Please note that storing the reagents at temperatures above -20°C makes them more prone to degradation when contaminated during use, and therefore storage at such temperatures is at the user′s own risk.

Quality

KAPA dNTPs are >99% pure by HPLC and are free of DNase, RNase, protease, phosphatase, and nicking activity. All kit components are subjected to stringent functional quality control, and meet strict requirements with respect to DNA contamination.