Not available outside of the UK & Ireland.

Application

KAPA Taq ReadyMix^™ has been used in:high throughput PCR amplification of low copy DNA templatesmultiplex PCRspecific amplification of complex templatesRT-PCRas a component of the reaction mixture for polymerase chain reaction (PCR) and for cDNA amplification

Biochem/physiol Actions

KAPA Taq and KAPA Taq HotStart^® DNA Polymerase elicit 5’;-3′ polymerase and 5′-3′ exonuclease activities, but no 3′-5′ exonuclease (proofreading) activity. The enzymes exhibit an error rate of approximately 1 error per 2.2 X 10⁵ nucleotides incorporated. In hot start formulation, the KAPA Taq associates with a proprietary antibody that inactivates the enzyme until the first denaturation step, eliminating spurious amplification products and increasing reaction efficiency and sensitivity. PCR products generated with KAPA Taq are A-tailed and are suitable for cloning into TA cloning vectors.

Features and Benefits

High performance: Improved sensitivity, specificity, and yields Novel buffer formulation facilitates specific primer annealing, leading to higher yield of specific productQuick Notes: KAPA Taq ReadyMix can replace any commercial Taq DNA polymerase in an existing protocol. The annealing temperature may need to be optimized to account for differences in formulation. The KAPA Taq PCR system is suitable for the amplification of fragments up to 3.5 kb from genomic DNA or 5 kb from less complex targets. The 2X KAPA Taq ReadyMix with dye includes two inert tracking dyes, which allow loading of PCR products directly onto agarose gels for analysis. KAPA Taq ReadyMixes contain 1.5 mM MgCl₂ and 0.2 mM of each dNTP (at 1X).

General description

KAPA Taq ReadyMix^™ (2X) is a ready-to-use cocktail containing all components for polymerase chain reaction (PCR), except primers and template. KAPA Taq ReadyMix is available with and without dye. The 2X ReadyMix with dye contains two inert tracking dyes to enable direct loading of PCR products onto agarose gels for analysis by electrophoresis, without the need to add a DNA loading solution. KAPA Taq DNA Polymerase is based on the single-subunit, wild-type Taq DNA polymerase of the thermophilic bacterium Thermus aquaticus.

Legal Information

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

HOTSTART is a registered trademark of Molecular BioProducts, Inc.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Preparation Note

Always ensure that the product has been fully thawed and mixed before use. Reagents may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for longterm storage

Quality

Each batch of KAPA Taq DNA Polymerase is confirmed to contain ﹤2% contaminating protein (Agilent Protein 230 Assay). KAPA Taq ReadyMixes are subjected to stringent quality control tests, are free of contaminating exo- and endonuclease activity, and meet strict requirements with respect to DNA contamination levels.