Not available outside of the UK & Ireland.

Application

The purified genomic DNA is ready for immediate use in downstream applications such as: restriction endonuclease digestions PCR Southern blots sequencing reactions cloning ligation

Features and Benefits

Expected yield: 25 µg from 2 x 10⁶ cultured cells; 30 µg from 25 mg of tissue Elution volume: 200 - 400 µl Time required: 20 min after lysis A₂₆₀/A₂₈₀ ratio: 1.6 - 1.9 Mechanical homogenization required: No

General description

The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian sources. These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.Isolate pure genomic DNA from cultured cells, tissues (including rodent tails), and fresh whole blood or white blood cells. The kit combines the advantages of silica binding with a microspin format and eliminates the need for expensive resins, alcohol precipitation, and hazardous organic compounds such as phenol and chloroform.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Other Notes

For additional information, please see www.sigma-aldrich.com/genomicdna.

Principle

The starting materials are lysed in a chaotropic salt-containing solution to ensure the thorough denaturation of macromolecules. Addition of ethanol causes DNA to bind when the lysate is spun through a silica membrane in a microcentrifuge tube. After washing to remove the contaminants, the DNA is eluted in 200 µL of a Tris-EDTA solution. The expected yields of genomic DNA will vary depending on the amount and type of starting material used. DNA purified with the kit has an A₂₆₀/A₂₈₀ ratio between 1.6 and 1.9 and can be up to 50 kb in length.