Not available outside of the UK & Ireland.

Application

Endotoxin Removal solution has been used to remove lipopolysaccharide (LPS) from Baker’;s yeast RNA.

Biochem/physiol Actions

The reagent relies on the micellar properties of Triton X-114 to remove endotoxins from solution. When warmed above 20 °C, micelles aggregate into a separate phase that sequesters amphiphilic solutes, including lipopolysaccharides. The hydrophilic DNA remains in the aqueous phase.

Quantity

1 volume treats 5 volumes of DNA solution (≤1mg/ml)