Not available outside of the UK & Ireland.

Application

JumpStart^™Taq Antibody is suitable for use in:cytochrome blocked nucleic acid (LNA) probe assayligation-mediated polymerase chain reaction (LM-PCR) in Real-Time quantitative PCR Analysis of viral titerPCR amplification of complex genomic or cDNA templatesto amplify very low-copy-number targetsfor the PCR amplification of many thermal cycles (>35) and multiple primer pairs in the same reaction tube

Components

JumpStart Taq Antibody, 1.1µg/1µLDilution Buffer provided in 1 mL vials

General description

JumpStart^™ Taq Antibody provides an efficient method for hot-start PCR. This antibody is a neutralizing monoclonal antibody specific to Taq DNA polymerase. When the antibody is bound to Taq, the enzyme is rendered inactive. At the first denaturation step in thermal cycling, the enzyme-antibody complex dissociates, and the JumpStart Taq Antibody becomes nonfunctional. Once the antibody is denatured, the activity of the Taq DNA polymerase is restored and the enzyme functions normally during the PCR reaction. JumpStart Taq Antibody is commonly used to prevent undesirable amplification products.

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC

Other Notes

View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.

Packaging

Supplied with dilution buffer.

Quantity

Two units of Taq DNA polymerase are inactivated by 1 test of JumpStart Taq Antibody.