Not available outside of the UK & Ireland.

Application

The Rapid DNA Dephos & Ligation Kit minimizes the time needed to perform diverse applications, including: Cloning of fragments into either plasmid or phage vectorsLinker ligationRecircularization of linear vector DNAGeneration of libraries

General description

The Rapid DNA Dephos & Ligation Kit enables fast and efficient dephosphorylation and ligation of sticky- or blunt-end DNA fragments.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Packaging

1 kit containing 5 components

Specificity

Specificity: rAPid Alkaline PhosphataseAlkaline Phosphatase catalyzes the hydrolysis of numerous phosphate esters, such as esters of primary and secondary alcohols, saccharides, cyclic alcohols, phenols and amines. Phosphodiesters do not react. The enzyme hydrolyzes inorganic pyrophosphate. The kinetic properties of the enzyme depend on many factors, such as purity of enzyme, concentration of enzyme in the assay, buffer, pH etc.Heat inactivation: rApid Alkaline Phosphatase:Two minutes at 75 °C.T4 DNA Ligase:10 minutes at 65 °C.

Unit Definition

Unit Definition: rAPid Alkaline PhosphataseOne unit of rAPid Alkaline Phosphatase is the enzyme activity which hydrolyzes one µmol of 4-nitrophenyl phosphate in one minute at +37 °C under assay conditions.Volume Activity: rApid Alkaline Phosphatase: 1 U/µlT4 DNA Ligase: 5 U/µl