Not available outside of the UK & Ireland.

Application

dUTP, PCR Grade, is suitable for many applications where high-quality reagents are required. Avoid carryover contamination between PCRs to eliminate a source of false positives.

General description

dUTP, PCR Grade, is a 100 mM clear, colorless solution of the sodium salt (pH 8.3).

Other Notes

For general laboratory use.

PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

Physical form

solution of dUTP water (pH 8.3).

Preparation Note

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP (10 mM, each), dUTP (30 mM); (e.g., for the preparation of 100 µl nucleotide mix add 10 µl of dATP, dCTP, dGTP (each) and 30 µl dUTP to 40 µl Water,PCR grade).

Quality

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.