Not available outside of the UK & Ireland.

Application

Streptavidin Mutein Matrix: Is highly suitable for purification of biotinylated proteinsHas been tested for use with in vitro expressed biotinylated proteins, using the RTS AviTag Biotinylation KitsIs convenient for purification of chemically biotinylated proteinsIt was also used in single-step affinity chromatography and affinity chromatography.

Features and Benefits

Mild purification conditions: Low biotin dissociation constant allows elution of biotinylated proteins under gentle conditions. Stable: No significant loss of binding capacity upon storage. The low dissociation constant of the streptavidin mutein is obtained by genetic engineering and not by dissociation of avidin- or streptavidin tetramers. Economic: High binding capacity and multiple reuse allows economic handling of the matrix.

General description

The binding of biotin to avidin/streptavidin is the strongest known noncovalent interaction of biological molecules [(K_d 0.6 x 10^-15 M (avidin), 4 x 10^-14 M (streptavidin)]. Strong binding provides a great advantage for certain applications of the derived biotin-avidin/streptavidin technology, such as surface immobilization, blotting technologies, immunoassays, cross-linking studies, and histochemistry. However, the use of immobilized avidin/streptavidin for the purification of biotinylated proteins is limited. In this instance, the extreme affinity of the binding partners creates a drawback of enabling elution only under very harsh conditions that usually destroy the functionality of the protein of interest.Recombinant streptavidin technology was used to screen for a mutant streptavidin with reduced binding affinity toward biotin. By substituting three amino acids, a mutein was obtained with a biotin dissociation constant of 1.3 x 10^-7 M.The Streptavidin Mutein Matrix provides this mutein immobilized onto cross-linked agarose beads in a highly stable and regenerable form, which allows purification of biotinylated proteins that results in excellent purity and recovery.

ContentsSettled resin of 50% suspension Streptavidin Mutein Matrix.

Streptavidin mutein, immobilized.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage and Stability

Store at 2 to 8 °C. (Do not freeze!)