Taq DNA Polymerase is a single subunit enzyme purified from the thermophilic bacterium Thermus aquaticus, the native enzyme expressed in E. coli. It polymerizes DNA from a primer annealed to a DNA template in the presence of dNTP for PCR.
- Highly purified recombinant enzyme
- Excellent batch-to-batch reproducibility
- Each lot is tested to be free of nuclease contamination
Taq DNA Polymerase (cloned) is the recombinant (segments of DNA from different sources joined together) form of the native enzyme, expressed in E. coli. This serves as a source of energy for cellular reactions and is involved in signaling pathways.
As Taq DNA Polymerase (cloned) is an exceptionally pure recombinant protein, it provides excellent batch-to-batch reproducibility, at an optimum temperature of 75°C, with the ability to survive repeated incubations at ﹥95°C.
Taq DNA Polymerase is licensed for use in PCR and is extensively tested for contaminating nickase, single- and double-stranded exonuclease, and endonuclease activities. When used with the supplied 10X PCR Buffer, the enzyme can successfully amplify single-copy genes from genomic DNA and can yield specific PCR products exceeding 2kb in length.
- Highly purified recombinant enzyme
- Excellent batch-to-batch reproducibility
- Each lot is tested to be free of nuclease contamination
This product has met the following criteria: