Not available outside of the UK & Ireland.

Application

The enzyme solution contains terminal deoxynucleotidyl transferase from calf thymus, recombinant in E. coli, needed to perform the TUNEL reaction for detecting apoptosis in situ.The TUNEL reaction mixture is used to label DNA strand breaks for detecting and quantitating apoptotic cell death at the single-cell level in cells and tissues.

General description

TUNEL enzyme solution, containing terminal deoxynucleotidyl transferase from calf thymus recombinant in E. coli.One of the two components required for performing the TUNEL reaction to detect apoptosis in situ.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Terminal deoxynucleotidyl transferase (TdT) in an optimized concentration, dissolved in reaction buffer. Ready-to-use with TUNEL Label.

Preparation Note

Working solution: TUNEL Enzyme is used in combination with TUNEL Label to prepare the TUNEL reaction mixture.To produce a TUNEL solution sufficient for one test: Mix 5µl TUNEL Enzyme with 45µl TUNEL Label prior to use. For negative control use 50µl/test TUNEL Label only.Storage conditions (working solution): Note: The TUNEL reaction mixture (45µl TUNEL Label with 5µl TUNEL Enzyme for 1 test) should be prepared immediately before use and should not be stored. Keep TUNEL reaction mixture on ice until use.