Not available outside of the UK & Ireland.

Application

TUNEL Label Mix has been used for the determination of apoptosis using TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) assay and DNA nick-end labeling by the TUNEL method.

The nucleotide-labeling mix (TUNEL Label) contains fluorescein-dUTP and -dNTPs, both needed to perform the TUNEL reaction for the detection of apoptosis in situ. The nucleotide-labeling mix is used in combination with the TUNEL Enzyme to prepare a TUNEL reaction mixture. This reaction mixture is used to label DNA-strand breaks for detecting and quantifying apoptotic cell death at a single-cell level in cells and tissues.

General description

The nucleotide-labeling mix (TUNEL Label) contains fluorescein-dUTP and -dNTPs, both needed to perform the TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) reaction for the detection of apoptosis in situ. The nucleotide-labeling mix is used in combination with the TUNEL enzyme to prepare a TUNEL reaction mixture. This reaction mixture is used to label DNA-strand breaks for detecting and quantifying apoptotic cell death at a single-cell level in cells and tissues.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working solution: TUNEL Label is used in combination with the TUNEL Enzyme to prepare the TUNEL reaction mixture.For one test: Mix 45 µl TUNEL Label with 5 µl TUNEL Enzyme prior to use. For negative control use 50 µl/test TUNEL Label only.Storage conditions (working solution): Note: The TUNEL reaction mixture (45 µl TUNEL Label with 5 µl TUNEL Enzyme for 1 test) should be prepared just before use, and should not be stored. Keep the TUNEL reaction mixture on ice until use.