Not available outside of the UK & Ireland.

Application

Use Streptavidin, recombinant for coating of solid phases (e.g., microplates, beads etc.) as well with biotin-labeled haptens and antibodies in universal immunological detection systems (streptavidin/biotin system).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

White lyophilizate; lyophilized in a 20 mM potassium phosphate, pH 6.5 solutionProtein: 0.7 ± 0.1mg protein lyophilizate (A₂₈₂ = 3.1) K_D: 10^-15M

Reconstitution

Add 1 ml double-distilled water to get a final concentration of 1 mg/ml (11 721 666 001) or 5 mg/ml (11 721 674 001), respectively.

Sequence

Streptavidin is a tetramer with a molecular weight of approximately 53 kD consisting of four subunits of approximately 13 kD, each containing a single biotin-binding site.Each subunit has six tyrosine residues.The protein is free of carbohydrate.

Unit Definition

One unit binds 1 µg D-biotin at pH 8.9.