Not available outside of the UK & Ireland.

Application

CSPD ready-to-use has been used for:the nonradioactive detection of labeled nucleic acid probes on membrane blots (Southern blot, Northern blot, Western blot and Dot blot)colony and plaque hybridizationgel shift assaysequencingIt may be used for the detection of alkaline phosphatase and alkaline phosphatase conjugates either in solution or on solid supports.

Features and Benefits

Save time with a fast, highly sensitive substrate. Easily strip and reprobe membranes. Take multiple exposures of up to two days for each experiment. Reuse the substrate by filtering and storing in sodium azide.

General description

CSPD is a chemiluminescent substrate for alkaline phosphatase that enables extremely fast and sensitive detection of biomolecules by producing visible light. Light emission is recorded on X-ray film or on luminescence imager systems.

Other Notes

For life science research only. Not for use in diagnostic procedures.Purity: Purified by HPLCDevelopment time: VariableNote: First exposure to X-ray film should be 15 to 30 minutes. Examine film and then determine other exposure times based on initial result.Activated by preincubation (10 minutes at +37°C).Apply substrate quickly to avoid drying of membrane (background).Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.Do not use nitrocellulose membranes.Repeat exposures can be made several days after addition of substrate.

Physical form

25mM solution (11.6mg/ml), 100x concentrated, colorless solution.

Preparation Note

Storage conditions (working solution): Washing Buffer: 15 to 25 °CMaleic Acid Buffer: 15 to 25 °CDetection Buffer: 15 to 25 °CBlocking Stock Solution, 10x: 2 to 8 °CBlocking Solution: always prepare freshAntibody Solution: 2 to 8 °C