Not available outside of the UK & Ireland.

Application

The DIG Wash and Block Buffer Set is used at various steps of DIG hybridization and DIG detection.

General description

Except for the Blocking solution, the 10x concentrated buffers are diluted to the desired concentrations with sterile double-distilled water (not included). Washing, blocking and detection buffers (10x concentrated) for the immunological detection of DIG - (digoxigenin-)labeled probes.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Packaging

1 set containing 4 components.

Preparation Note

Working solution: dATP, dGTP, dTTP mixture:For one labeling reaction pipette:1 μl dATP, (vial 2)1 μl dGTP, (vial 4)1 μl dTTP, (vial 5)to a reaction vial.Note: If the same type of labeled deoxyribonucleoside-triphosphate is used repeatedly, we recommend the preparation of a mixture of equal parts of the other three deoxyribonucleoside-triphosphates for convenience.Storage conditions (working solution): •After the first usage, the buffers should be stored further at 2 to 8 °C. We recommend to store the concentrated Blocking solution in aliquots at -15 to -25 °C.•When Autoclaved: Stock solution can be stored for several days to a week either unopened at RT or at 2 to 8 °C after opening. Alternatively, it can be stored in aliquots at -15 to -25 °C for up to 6 months.

Quality

The buffers are autoclaved, filtered and tested for the absence of DNases and RNases according to the current Quality Control procedures. The buffers are function tested according to the standard procedure of the DIG Nucleic Acid Detection Kit.