Not available outside of the UK & Ireland.

Application

The ready-to-use solution is directly added to the hybridization mix. It prevents nonspecific hybridization in all types of membrane (e.g., Southern and northern blot applications) or in situ DNA hybridizations. DNA, MB-grade has been used in chromatin immunoprecipitation (ChiP) assay.Note: Addition of NaCl, sonication or shearing, and denaturing are not required.

General description

DNA, MB-grade is used for the prevention of nonspecific hybridization reactions.

DNA, MB-grade, is provided as a sonicated, denatured single-stranded DNA fragment mixture.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Solution, 10 mg/ml, in 10 mM Tris-HCl, 10 mM NaCl, 1 mM EDTA, pH 8.0

Preparation Note

Working concentration: 100µg/mlThis fish sperm DNA should be used at a concentration of 100 µg/ml (1 : 100 dilution) in prehybridization solutions.

Sequence

Chain Length 120 to 3,000 nucleotides