Not available outside of the UK & Ireland.

Application

Colorimetric AP substrate for: ImmunohistocytochemistryWestern blotSouthern blotNorthern blotColony and plaque hybridizationIn situ hybridization

Components

Ready-to-use solution.

General description

BM purple is a chromogenic substrate for alkaline phosphatase (AP) designed for precipitating enzyme immunoassays. It develops a permanent, dark purple band or spot at the AP binding site on the membrane or solid support. The stabilized substrate solution may be used directly from the bottle with no mixing or reconstitution. It gives less background staining than when using the BCIP/NBT two-bottle system, without a loss of sensitivity. The reaction product has a dark purple color and is insoluble in water. The dark purple color can be visually seen.Appearance prior to use: light yellow color; After color development: dark purple

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working concentration: Best results have been obtained using 200 mU POD and casein as blocking reagent.Working solution: Solubility in water: insoluble.