Not available outside of the UK & Ireland.

Application

Use Endoproteinase Glu-C Sequencing Grade for protein structure analysis and for sequence analysis.

Features and Benefits

Free of impurities that may interfere with separation of peptides in RP HPLC.

General description

Endoproteinase Glu-C Sequencing Grade is a serine protease isolated from Staphylococcus aureus V8 as a highly purified and specific protease. Inhibitors of Endoproteinase Glu-C are DFP, α2-macroglobulin and TLCK.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working concentration: 1/100 to 1/20 (w/w)With a minimum amount of 1 to 5 µg insulin B_ox, sufficient levels can be detected using the Column Aquapore RUP 300, 4.6 x 100 nm, 7 µm.Working solution: Solvent is recommended in double-distilled water.Solvents other than water have not been tested; Roche recommends using 50 mM Hepes, pH 7.8.Storage conditions (working solution): +2°C to +8°C for 1-2 days.Stability: At +2 to +8°C within specification range for 12 months; stored dry.

Reconstitution

Lyophilized Endoproteinase Glu-C sequencing grade is reconstituted in double-dist. water. To avoid autolysis, the incubation temperature should not exceed 25 °C.

Specificity

Serine protease that very specifically hydrolyzes peptide bonds at the carboxylic side of Glu (in ammonium bicarbonate, pH 7.8, or ammonium acetate buffer, pH 4.0), or Glu and Asp (in phosphate buffer, pH 7.8). The specificity of Endoproteinase Glu-C Sequencing Grade is verified with the oxidized B-chain of insulin (insulin B_ox) as substrate. High concentrations of Endoproteinase Glu-C Sequencing Grade (1 part by weight enzyme with 10 parts by weight insulin B_ox) are incubated to detect traces of impurities, for example, contaminating proteases.Heat inactivation: Most proteases sequencing grade are stopped by boiling for 5 minutes.