Not available outside of the UK & Ireland.

Application

Biotin-labeled RNA can be used as a hybridization probe for:Northern blotsSouthern blotsPlaque or colony liftsRNase protection experimentsIn situ hybridizationMicroarray hybridizationThe labeled RNA can be subsequently detected with a fluorescent streptavidin conjugate or by enzyme linked immuno sorbent assay (ELISA) using a Streptavidin-AP conjugate.

General description

For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling. Linearized template DNA with T7, SP6, or T3 promoter is in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Biotin-16-UTP, tetralithium salt, 10mM solution

Quality

Typical analysis: At least 85% Biotin-16-UTP (HPLC, area%).