Not available outside of the UK & Ireland.

Application

Fluorescein-12-dUTP is used as a substrate for: terminal transferaseDNA polymerase I (holoenzyme and Klenow fragment),Taq DNA polymerasereverse transcriptase (e.g., from AMV and M-MuLV).Fluorescein-12-dUTP has been used to label nuclear extracts during PCR. It has also been used to replace dTTP in the random-primed DNA labeling reaction or in nick translation reactions, as well as in PCR. The nucleotide also serves as a substrate for terminal transferase in 3′-end labeling and TUNEL. Fluorescein-labeled probes can be used for in in situ hybridization with direct fluorescence detection or detection by enzyme linked immunoimmunsorbent assay (ELISA) using Anti-Fluorescein-AP, Fab fragments. Repeated fluorescence labeling using Tetramethyl-Rhodamine-6-dUTP (red) and AMCA-6-dUTP (bright blue) is possible.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Fluorescein-12-dUTP, tetralithium salt.

Quality

Typical analysis: At least 85% Fluorescein-12-dUTP (HPLC, area%).

Storage and Stability

The unopened reagent is stable at 15 to 25°C until the expiration date printed on the label. Decomposition of approx. 5% may occur within 6 months.