Not available outside of the UK & Ireland.

Analysis Note

Biological activity: Assayed for high cloning efficiency of a hybridoma cell line.

Application

Hybridoma Fusion and Cloning Supplement (HFCS) is used as a supplement to the normal culture medium to support the growth of B-cell hybridomas after fusion and during cloning. HFCS replaces feeder cells. It is also used to optimize the growth of hybridomas after the thawing of cells stored in liquid nitrogen.

General description

Medium supplement composed of albumin, insulin, transferrin, cytokines, a cholesterol source, other defined organic and inorganic compounds and fetal calf serum (final concentration: 0.5%).HFCS contains human proteins. The raw material from which the human proteins were isolated, has been tested for the presence of Hepatitis B surface Antigen (HBsAg) and HIV-1/2 antibodies and found to be negative (according to current quality control procedures).

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Solution (50x concentrated, pH 7.4); filtered through 0.2 µm pore size membrane

Preparation Note

Working concentration: HFCS (50x) is diluted 1:50 (v/v) with basal medium. It is strongly suggested that you use RPMI 1640. The final medium should also contain L-glutamine and sodium bicarbonate.