Not available outside of the UK & Ireland.

Application

DNA Molecular Weight Marker II, DIG-labeled has been used as a size standard in Southern blot analysis for nucleic acid labeling and detection.

Features and Benefits

ContentsReady-to-use solutions in 10mM Tris-HCl, 1mM EDTA, pH 8.0.

General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

DNA Molecular Weight Marker II, DIG-labeled is a solution in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0. This fragment mixture is prepared by cleavage of λDNA with Hind III. The size of the fragment ranges from 0.12 to 23.1 kbp. A photodigoxigenin has been introduced at approx. every 200th to 300th base in the DNA fragments.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Sequence

The mixture contains 8 fragments with the following base pair lengths: 125, 564, 2027, 2322, 4361, 6557, 9416, and 23130 bp.Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.