ROCHE Streptavidin-POD conjugate

Not available outside of the UK & Ireland.

Application

Streptavidin-POD has been used for: ELISAIn situ hybridizationWestern blotIt is also used for immunohistochemistry.

General description

Streptavidin is covalently coupled to horseradish peroxidase. The conjugate is used for detection of biotin-labelled substances.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Lyophilizate, stabilized

Preparation Note

Working concentration: 5 - 500 mU/ml (i.e., stock solution can be diluted). Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:•ELISA: 5 to 50 mU/ml•Immunohistocytochemistry: 250 to 500 mU/ml•Western blot: 100 to 250 mU/ml

Reconstitution

Add 1 ml double-distilled water to a final concentration of 500 U/ml.The reconstituted stock solution is stable at 2 to 8 °C for 6 months.Do not freeze reconstituted antibody! Do not add sodium azide!A further dilution of reconstituted stock solution in double-distilled water is stable for approx. one week at 2 to 8 °C.

Unit Definition

One unit Streptavidin-POD, corresponding to one unit peroxidase, will oxidize 1 µmol of guaiacol tetramer in one minute at +25 °C and pH 7.0.Unit Conversion: 1 U (+25 °C; guaiacol as substrate; pH7.0) corresponds to 1.24 U (+25 °C; 1.7 mM ABTS as substrate; pH= 6.0) or to 4.0 U (+25 °C; 8.7 mM ABTS as substrate; pH = 5.0).