BM Condimed H1 (Hyb.-Cloning-Suppl.)

Not available outside of the UK & Ireland.

Analysis Note

Biological activity: Assayed for its ability to promote the growth of freshly fused hybridoma cells.

Application

BM Condimed H1 is used as a supplement to the normal culture medium to support the growth of B-cell hybridomas after fusion and during cloning. BM Condimed H1 replaces feeder cells. It is also used to optimize the growth of hybridomas after the thawing of cells stored in liquid nitrogen.

Cell Line Origin

BM Condimed H1 is prepared from the supernatant of a mouse lymphoma cell line stimulated with PMA. It contains a complex mixture of growth factors and cytokines that have a marked stimulatory effect on the growth of hybridoma cells after fusion and during cloning.

Features and Benefits

ContentsSolution in RPMI 1640, filtered through 0.2µm pore size membrane

General description

BM Condimed H1 is prepared from the supernatant of a mouse lymphoma cell line stimulated with PMA. It contains a complex mixture of growth factors and cytokines that have a marked stimulatory effect on the growth of hybridoma cells after fusion and during cloning.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Solution in RPMI 1640, filtered through 0.2 µm pore size membrane

Preparation Note

Working concentration: 10% (v/v) in culture medium that contains 10 - 20% FBS.