Not available outside of the UK & Ireland.

Application

Fibronectin promotes the attachment and subsequent spreading of many cells. It is used for the coating of culture dishes. It has been used for the cell adhesion assay.

General description

Fibronectin is responsible for the cellular interactions with the extracellular matrix. It is involved in cell adhesion, migration, growth and differentiation. It is a soluble component of plasma and other body fluids. In addition, it is also part of the insoluble extracellular matrix.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Lyophilizate containing 1.126 mg glycine and 0.058 mg sodium chloride per mg fibronectin

Preparation Note

Working concentration: 5µg/cm² for the coating of cell culture vesselsStorage conditions (working solution): -15 to -25 °CThe reconstituted solution is stable at -15 to -25 °C.Note: Store the reconstituted solution in aliquots at -15 to -25 °C.Avoid repeated freezing and thawing.

Reconstitution

Add 1 or 5 ml sterile water, respectively, to yield a final concentration of 1 mg/ml. Incubate 30 to 60 minutes at 37 °C to dissolve. Do not agitate.Upon reconstitution, the solution may contain a small amount of insoluble aggregated material. These aggregates are a phenomenon inherent to fibronectin and do not influence product performance.

Sequence

Human plasma fibronectin consists of two similar polypeptide chains ( α-, β-chain, each of 220 kDa), connected by two disulfide bridges.

Specificity

Active on most mammalian cellsBiological activity: Tested for the promotion of adherence of 3T3 (NR6) cells (XTT cleavage).