Not available outside of the UK & Ireland.

Application

The NAG standard is used for the determination of NAG activity in urine in life science research applications, with the aid of N-acetyl-β-D-glucosaminidase. It can be used in automatic analyzers.

General description

Standard for the determination of NAG activity in urine research samples with the aid of the Test-Combination N-acetyl-β-D-glucosaminidase.N-Acetyl-β-D-Glucosaminidase is a high molecular weight lysosomal enzyme existing as two isofomrs, Isoenzyme-A and Isoenzyme-B. It is present in human kidney. It has high molecular weight. It is highly active in renal proximal tubular cells. It has been reported that overexpressed urine-NAG reflects the proximal tubular dysfunction of the kidney.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Storage conditions (working solution): 2 to 8 °CStability of the standard solutionThe solution is stable for at least 1 month stored at 2 to 8 °C, protected from light.

Reconstitution

Preparation of the standard solutionDissolve contents of one bottle with 3 ml double distilled water, close bottle and wait for 5 minutes. Dissolve contents completely by gentle swirling. Avoid creating foam.

Unit Definition

Volume Activity: The activity of the NAG standard is approximately 20 mU N-acetyl-β-D-glucosaminidase from beef kidney per ml. For exact values, see data printed on the label.