Not available outside of the UK & Ireland.

Application

BM-Cyclin is used for the elimination of mycoplasma from infected cell cultures without obvious marked cytotoxic side effects.

General description

BM-Cyclin is a combination of two antibiotics, namely tiamulin and minocycline, both of which prevent protein synthesis.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Packaging

Set of two different antibiotics

Physical form

Vial 1 (Tiamulin-hydrogenfumarat): clear, colorless solution after reconstitutionVial 2 (Minocyclinhydrochloride): clear, yellow solution after reconstitution

Preparation Note

Working concentration: 10µg/ml pleuromutilin derivative (BM-cyclin 1), 5µg/ml tetracycline derivative (BM-cyclin 2).Note: These concentrations do not affect the growth of most cells. Lower concentrations may be used for sensitive cell lines.For hybridomas decrease concentration of BM-Cyclin by 50%.Preparation of DAPI Working SolutionDilute the stock solution with methanol to a final concentration of 1µg/ml.Working solution: Preparation of Stock SolutionDissolve content of BM Cyclin 1 and BM Cyclin 2 in 10ml sterile PBS or water.Note: These are 250x concentrated stock solutions and can be stored at -15 to -25°C for at least 6 months.Preparation of DAPI Stock SolutionDissolve DAPI in water to a final concentration of 1 to 5mg/ml.Note: Do not use any buffers.This stock solution can be stored at -15 to -25°C. It is recommended to prepare appropriate aliquots.Storage conditions (working solution): -15 to -25°CReconstituted stable at -15 to -25°C for at least 6 months.

Specificity

Sensitive Organisms: Mycoplasmas and bacteria BM Cyclin was found to effectively eliminate Acholesplasma laidlawii, Mycoplasma arginini, Mycoplasma hyorhinis, and Mycoplasma oralefrom experimentally contaminated and chronically infected cell lines.Note: These mycoplasma strains account for more than 85% of the contaminations in animal cell cultures.