Not available outside of the UK & Ireland.

Application

Use Endoproteinase Glu-C (V8 Protease) for protein structure analysis and for sequence analysis.

Biochem/physiol Actions

Endoproteinase Glu-C specifically hydrolyzes peptide and ester bonds at the carboxylic side of Glu, or both Glu and Asp, depending on the buffer used.

General description

Endoproteinase Glu-C is a Staphylococcal serine proteinase. Its inhibitors are DFP, α2-macroglobulin and TLCK.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Activator: The enzyme has its maximal activity in presence of SH-reagentsWorking concentration: 1 to 5 mMWorking solution: Recommended solvent is 50 mM ammonium acetate pH 4.0 (2 mg/ml).Storage conditions (working solution): -15 to -25 °CThe enzyme (2 mg/ml in 50 mM ammonium acetate, pH 4.0) is stable for at least one month, frozen in aliquots and thawed only once.

Specificity

Heat inactivation: Endoproteinase Glu-C is inactivated by boiling for ten minutes.

Storage and Stability

Store at 2 to 8 °C. (Store dry!)