Not available outside of the UK & Ireland.

Application

Creatine kinase has been used as a component of translation buffer used in in vitro translation assay and as a component of energy regeneration mix.

Biochem/physiol Actions

Creatine kinase (CK) is a guanidino-kinase that catalyzes the reversible phosphorylation of creatine to phosphocreatine at the expense of ATP. It plays crucial roles in rapid regeneration of ATP in cells with high demand for energy. Increased level of CK in human blood indicates diseases of the nervous system and the heart muscle, malignant hypothermia, and certain tumors. Metals such as Mg, Mn and Ca act as cofactors for CK. It also forms a substrate for protein kinase C, an important signaling molecule involved in cell growth. A product of enzymatic reaction of CK, phosphocreatine, is found to prevent HIV (human immunodeficiency virus) replication in macrophages.

General description

ATP:creatine N-phosphotransferaseCreatine kinase (CK) has a molar mass of 85kDa. It exists as a dimer in the cytosolic form and as a dimer and an octamer in the mitochondrial form. The cytosolic forms are named as MM, BB, and MB, where M and B correspond to muscle and brain, respectively. CK from rabbit muscle is a dimer containing two identical polypeptide chains of 380 amino acids. Each subunit has four cysteines, Cys73, Cys145, Cys253 and Cys282, but lacks inter- and intra-chain disulfide bonds.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Lyophilizate, stabilized

Preparation Note

Storage conditions (working solution): Working solutions and storage conditions in routine applications:Dissolve in 0.1 M imidazole buffer; aliquot and store at -15 to -25 °C. Stable for approximately four weeks at -15 to -25 °C.Avoid repeated freezing and thawing.Dissolve in 0.5% NaHCO3 solution.Do not freeze. Store protected from light.Stable for 2 - 3 days, when stored at 2 to 8 °C.OrDissolve in 30 mM glycine buffer, pH 9.0, with 0.2 mM DTT.Stable for 2 - 3 days, when stored at 2 to 8 °C.

Quality

Contaminants: ﹤0.001% ATPase, HK, and myokinase, each

Specifications

Specific activity: Approximately 350U/mg lyophilizate at +25°C (800U/mg lyophilizate at +37°C) with creatine phosphate and ADP as the substrates, and N-acetyl-L-cysteine as activator.

Unit Definition

One unit CK activity (+25 °C) = 2.3 U (+37 °C), creatin-P and ADP as substrates, NAcCys as reactivator.