ROCHE Isopropyl-beta-D-thiogalactoside

Not available outside of the UK & Ireland.

Application

IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

Isopropyl-β-D-thiogalactoside (IPTG) is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. It has been used for the expression of recombinant genes in E. coli.

General description

Isopropyl-β-D-thiogalactoside (IPTG) is a chemical analog of galactose that cannot be cleaved by β-galactosidase. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

Other Notes

For life science research only. Not for use in diagnostic procedures.

The amount of labeled DNA depends on: amount of template DNA purity of template DNA conformation of template DNA average size of labeled fragment

Non-metabolizable galactose analog.

Preparation Note

Working concentration: Final concentration should be 0.2 mM.Storage conditions (working solution): Stock solutions of the product in water (e.g., 1 M IPTG) stored at -15 to -25 °C, are stable up to six months.

Sample Material 10 ng to 3µg template DNA, linearized and at least 100 to 200bp long

Specifications

Assay Time: 50minutesSpecific Activity: The described standard assay will results in a specific activity of 1.8 x 10⁹ dpm/µg, corresponding to 65% incorporation with different substrate DNAs in 30minutes. When varying the ratio of template DNA to labeled dNTP, similar incorporation rates, but different levels of specific activity of the labeled probe are obtained.