Not available outside of the UK & Ireland.

Application

Use Chromozym^® PK as a substrate for the determination of serine proteases, specifically plasma kallikrein in citrated plasma.

General description

Chromozym^® PK is a non-radioactive amidolytic chromogenic substrate mostly used in kinetic analysis of plasma kallikrein.

Legal Information

Chromozym is a registered trademark of Pentapharm AG, Basel

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Activator: Mg²⁺ (K_m = 3.0 mM, pH 7.4, 25 °C); other divalent metal ions (Mn²⁺, Fe²⁺, Zn²⁺)Working concentration: Approximately 0.5 mMStorage conditions (working solution): 2 to 8 °CAn aqueous solution of Chromozym PK (1.3 mM) is stable for at least 4 weeks if stored at 2 to 8 °C.

Principle

Chromozym PK is cleaved by plasma kallikrein into a residual peptide and free 4-nitraniline, which is measured at 405 nm. The absorbance difference per minute is used for the determination of the kallikrein activity in U/ml.

Quality

Contaminant: ﹤0.5% free 4-nitraniline

Sequence

Benzoyl-prolyl-phenalanyl-arginine-4-nitranilide-acetate