Not available outside of the UK & Ireland.

Application

5-Bromo-2′-deoxyuridine has been used to label cells in the S-phase of the cell cycle and primary hepatocytes during cell proliferation studies.

Immunochemically detectable nucleotide that can be incorporated into DNA instead of thymidine. Used for in vivo studies of DNA synthesis and for the detection of mutagenic substances, according to the sister chromatid exchange (SCE) method.

Biochem/physiol Actions

Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.

General description

It is conventionally used as a marker for S-phase synthesis of DNA.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Preparation Note

Working concentration: According to literature for cell culture, a final concentration of 10 µmol/l has been used.According to literature, 5 to 50 mg/kg body weight mice have been used for the detection of cell proliferation.Preparation of Working SolutionCorresponding to the BrdU-labeling reagent which is included in the Roche Cell Proliferation ELISA, BrdU kits and in the Roche 5-Bromo-2′-deoxy-uridine Labeling and Detection Kits, prepare the BrdU working solution by dissolving the substance in PBS to a 10 mM stock solution (MW of BrdU = 307.1 D). For the in vivo use of BrdU dissolve in PBS; for the in vitro use of BrdU, dissolve in double-distilled water at the same 10 mM concentration.Storage conditions (working solution): -15 to -25 °C

Quality

Purity: 97% (from N), chromatographically homogeneous