Not available outside of the UK & Ireland.

Application

Hexokinase/Glucose-6-phosphate dehydrogenase has been used for the determination of plasma glucose levels.

Components

EC 2.7.1.1 & 1.1.1.49

General description

Hexokinase is responsible for catalyzing the first step of glycolysis. It results in the formation of glucose-6-phosphate and ADP from glucose and ATP. Glucose-6-phosphate dehydrogenase is a crucial enzyme in the pentose phosphate pathway. It is responsible for the conversion of glucose-6-phosphate and NADP+ (nicotinamide adenine dinucleotide phosphate) to 6-phosphogluconolactone and NADPH.

Other Notes

For life science research only. Not for use in diagnostic prodcedures.

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6. Prepared by mixing hexokinase with G6P-DH. HK:G6P-DH approximately 2:1 with regard to protein content.

Unit Definition

One unit (U) HK will phosphorylate 1 µmol of D-glucose in one minute at +25 °C and pH 7.6.One unit (U) G6P-DH will oxidize 1 µmol of glucose-6-phosphate in one minute at +25 °C and pH 7.6.The coupled assay produces 1 µmol of NADH per µmol of D-glucose phosphorylated.Volume Activity: 340 U hexokinase/ml at +25 °C with glucose and ATP as the substrates. 170 U glucose-6-phosphate dehydrogenase/ml at +25 °C with glucose-6-phosphate as the substrate